BillionToOne

BillionToOne is a precision diagnostics company based in Menlo Park, California, founded in 2016. With a team of around 300 employees, the company aims to enhance molecular diagnostics by making them more accurate, efficient, and accessible. Their proprietary technology, Quantitative Counting Templates (QCTs™), allows for the detection and measurement of disease-related DNA fragments at single base-pair resolution, significantly improving prenatal screening and liquid biopsy applications. The company's flagship product, UNITY™ Screen, is a noninvasive prenatal test that analyzes fetal cell-free DNA from a maternal blood sample, assessing risks for various genetic conditions and chromosomal aneuploidies. BillionToOne also offers UNITY Complete®, which combines multiple screening options into one test. In oncology, they provide liquid biopsy tests like Northstar Select™ and Northstar Response™ to help determine cancer treatment and monitor therapy effectiveness. Additionally, they have developed a COVID-19 test, showcasing their technology's versatility. BillionToOne serves pregnant patients, oncology patients, and clinical research organizations, and has experienced significant growth and recognition in the diagnostics field.

Research Menlo Park, United States Founded 2016 ~484 employees Private · Series D

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Employees
484
+5.9% in the 3 months to Jun 2025
Revenue / employee
$316K
Revenue ~$153M
Total funding
$423M
Last round Series D, Jun 2024
12-month attrition
8.2%
Tech: 12.9%
H1B filings, FY2026
9
Sponsors visas
Open jobs
36
Last 30 days

H-1B sponsorship

H-1B Sponsorship Limited
H-1B Filings by Department
Engineering18
Healthcare4
Executive1

The department split adds up all the years listed above. FY2026 alone: 9 filings.

Funding

  • Jun 2024 · Series D · $130M · Premji Invest, Neuberger Berman, Adam Street Partners
  • Dec 2022 · Series C · $48M · Libertus Capital, Hummingbird Ventures, Baillie Gifford
  • Dec 2022 · Debt Financing · $35M · Libertus Capital, Hummingbird Ventures, Baillie Gifford
  • Mar 2022 · Series C · $125M · Adams Street Partners, Hummingbird Ventures, Baillie Gifford
  • Jun 2021 · Series B · $55M · Hummingbird Ventures, Four Rivers Group, Neotribe Ventures
  • Mar 2020 · Series A · $15M · Hummingbird Ventures, NeoTribe Ventures, Y Combinator
  • Mar 2019 · Series A · $15M · Hummingbird Ventures, NeoTribe Ventures, Y Combinator

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BillionToOne FAQ

How many employees does BillionToOne have?

BillionToOne has about 484 employees, up 5.9% over the 3 months to Jun 2025.

Does BillionToOne sponsor H1B visas?

Yes. BillionToOne filed 9 H1B (LCA) applications in FY2026, according to the latest U.S. Department of Labor disclosures. Most of them were for Engineering roles.

What is BillionToOne's revenue per employee?

BillionToOne makes about $316K in revenue per employee, based on $153M annual revenue and 484 employees.

How much funding has BillionToOne raised?

BillionToOne has raised $423M in total funding; its latest round was Series D in Jun 2024.

Data from Company Prospectus, DOL LCA disclosures, WARN notices and 6figr salary profiles.