BillionToOne

BillionToOne is a precision diagnostics company based in Menlo Park, California, founded in 2016. With a team of around 300 employees, the company aims to enhance molecular diagnostics by making them more accurate, efficient, and accessible. Their proprietary technology, Quantitative Counting Templates (QCTs™), allows for the detection and measurement of disease-related DNA fragments at single base-pair resolution, significantly improving prenatal screening and liquid biopsy applications. The company's flagship product, UNITY™ Screen, is a noninvasive prenatal test that analyzes fetal cell-free DNA from a maternal blood sample, assessing risks for various genetic conditions and chromosomal aneuploidies. BillionToOne also offers UNITY Complete®, which combines multiple screening options into one test. In oncology, they provide liquid biopsy tests like Northstar Select™ and Northstar Response™ to help determine cancer treatment and monitor therapy effectiveness. Additionally, they have developed a COVID-19 test, showcasing their technology's versatility. BillionToOne serves pregnant patients, oncology patients, and clinical research organizations, and has experienced significant growth and recognition in the diagnostics field.

Research Menlo Park, United States ~759 employees Private · Series D

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Employees
759
+1.7% in the 3 months to Sep 2026
Revenue / employee
$202K
Revenue ~$153M
Total funding
$423M
Last round Series D, Jun 2024
12-month attrition
8.8%
Tech: 7.6%
H1B filings, FY2026
9
Sponsors visas
Open jobs
61
Last 30 days

Headcount trend

BillionToOne went from 446 employees in Oct 2024 to 759 in Sep 2026. Apr to Sep 2026: 107 people joined, 22 left.

Org structure

Headcount by department

Other484
Sales98
Engineering68
Operations38
Success23
HR16
Finance12
Marketing11

H-1B sponsorship

H-1B Sponsorship Limited
H-1B Filings by Department
Engineering18
Healthcare4
Executive1

The department split adds up all the years listed above. FY2026 alone: 9 filings.

Funding

  • Jun 2024 · Series D · $130M · Premji Invest, Neuberger Berman, Adam Street Partners
  • Dec 2022 · Series C · $48M · Libertus Capital, Hummingbird Ventures, Baillie Gifford
  • Dec 2022 · Debt Financing · $35M · Libertus Capital, Hummingbird Ventures, Baillie Gifford
  • Mar 2022 · Series C · $125M · Adams Street Partners, Hummingbird Ventures, Baillie Gifford
  • Jun 2021 · Series B · $55M · Hummingbird Ventures, Four Rivers Group, Neotribe Ventures
  • Mar 2020 · Series A · $15M · Hummingbird Ventures, NeoTribe Ventures, Y Combinator
  • Mar 2019 · Series A · $15M · Hummingbird Ventures, NeoTribe Ventures, Y Combinator

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BillionToOne FAQ

How many employees does BillionToOne have?

BillionToOne has about 759 employees, up 1.7% over the 3 months to Sep 2026.

Does BillionToOne sponsor H1B visas?

Yes. BillionToOne filed 9 H1B (LCA) applications in FY2026, according to the latest U.S. Department of Labor disclosures. Most of them were for Engineering roles.

What is BillionToOne's revenue per employee?

BillionToOne makes about $202K in revenue per employee, based on $153M annual revenue and 759 employees.

How much funding has BillionToOne raised?

BillionToOne has raised $423M in total funding; its latest round was Series D in Jun 2024.

Data from Company Prospectus, DOL LCA disclosures, WARN notices and 6figr salary profiles.